Plasmid_Backbone

Part:BBa_K1680026:Design

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-18)


pETUE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 3053
    Illegal NgoMIV site found at 3213
    Illegal AgeI site found at 3270
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 374
    Illegal BsaI.rc site found at 1477


Design Notes

very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion



Source

pET-Vector

References